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diff quick stain kit  (Dade Behring)

 
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    Structured Review

    Dade Behring diff quick stain kit
    Diff Quick Stain Kit, supplied by Dade Behring, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diff+quick+kit/diff+quick/pm42162069-128-8-11
    Average 86 stars, based on 1 article reviews
    diff quick stain kit - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Staining:

    Article Title: HMGB1-stimulated human primary cardiac fibroblasts exert a paracrine action on human and murine cardiac stem cells.
    Article Snippet: HighMobility Box 1 Protein (HMGB1) is a cytokine released into the extracellular space by necrotic cells and activated macrophages in response to injury.. We recently demonstrated that HMGB1 administration into the mouse heart during acute myocardial infarction induces cardiac tissue regeneration by activating resident cardiac c-kit cells (CSCs) and significantly enhances left ventricular function.. In the present study it was analyzed the hypothesis that human cardiac fibroblasts (cFbs) exposed to HMGB1 may exert a paracrine effect on mouse and human CSCs.

    Article Title: A novel regulator of angiogenesis in endothelial cells: 5-hydroxytriptamine 4 receptor
    Article Snippet: .. After 4 h of incubation, the filter was fixed and stained with Diff Quick kit (Dade Behring, Newark, DE). ..

    Article Title: Identification of a small molecule(s) class to enhance cell-cell adhesion and attenuate prostate tumor growth and metastasis
    Article Snippet: .. The incubations were carried out for 24 hours, after which Matrigel TM (along with non-invading cells) was scraped off with cotton swabs, and outer side of the insert was fixed and stained using Diff Quick kit (Dade-Behring Diagnostics, Newark, DE). ..

    Article Title: Non-Destructive Biomarkers in Non-Target Species Earthworm Lumbricus terrestris for Assessment of Different Agrochemicals
    Article Snippet: .. Morphometric alterations of granulocytes were determined by image analysis of cells stained with the Diff-Quick® kit (Dade Behring, Newark, USA). ..

    Article Title: N-Cadherin and Keratinocyte Growth Factor Receptor Mediate the Functional Interplay between Ki -RAS G12V and p53 V143A in Promoting Pancreatic Cell Migration, Invasion, and Tissue Architecture Disruption
    Article Snippet: .. After 24 h, the cells that did not migrate or invade were removed from the upper faces of the inserts using cotton swabs, and the cells that migrated to or invaded the lower surfaces of the inserts were fixed in methanol and stained using a Diff-Quick kit (Dade Behring Inc., Newark, DE). ..

    Article Title: A macrophage migration inhibitory factor-like tautomerase from Teladorsagia circumcincta (Nematoda: Strongylida)
    Article Snippet: .. Filters were excised from the Transwell assembly, stained using a Diff Quick kit (Dade-Behring A.G., Duedingen, Switzerland) and mounted on glass slides in Mowiol® mountant (Calbiochem). ..

    Diff-Quik:

    Article Title: HMGB1-stimulated human primary cardiac fibroblasts exert a paracrine action on human and murine cardiac stem cells.
    Article Snippet: HighMobility Box 1 Protein (HMGB1) is a cytokine released into the extracellular space by necrotic cells and activated macrophages in response to injury.. We recently demonstrated that HMGB1 administration into the mouse heart during acute myocardial infarction induces cardiac tissue regeneration by activating resident cardiac c-kit cells (CSCs) and significantly enhances left ventricular function.. In the present study it was analyzed the hypothesis that human cardiac fibroblasts (cFbs) exposed to HMGB1 may exert a paracrine effect on mouse and human CSCs.

    Article Title: A novel regulator of angiogenesis in endothelial cells: 5-hydroxytriptamine 4 receptor
    Article Snippet: .. After 4 h of incubation, the filter was fixed and stained with Diff Quick kit (Dade Behring, Newark, DE). ..

    Article Title: Identification of a small molecule(s) class to enhance cell-cell adhesion and attenuate prostate tumor growth and metastasis
    Article Snippet: .. The incubations were carried out for 24 hours, after which Matrigel TM (along with non-invading cells) was scraped off with cotton swabs, and outer side of the insert was fixed and stained using Diff Quick kit (Dade-Behring Diagnostics, Newark, DE). ..

    Article Title: Non-Destructive Biomarkers in Non-Target Species Earthworm Lumbricus terrestris for Assessment of Different Agrochemicals
    Article Snippet: .. Morphometric alterations of granulocytes were determined by image analysis of cells stained with the Diff-Quick® kit (Dade Behring, Newark, USA). ..

    Article Title: N-Cadherin and Keratinocyte Growth Factor Receptor Mediate the Functional Interplay between Ki -RAS G12V and p53 V143A in Promoting Pancreatic Cell Migration, Invasion, and Tissue Architecture Disruption
    Article Snippet: .. After 24 h, the cells that did not migrate or invade were removed from the upper faces of the inserts using cotton swabs, and the cells that migrated to or invaded the lower surfaces of the inserts were fixed in methanol and stained using a Diff-Quick kit (Dade Behring Inc., Newark, DE). ..

    Article Title: A macrophage migration inhibitory factor-like tautomerase from Teladorsagia circumcincta (Nematoda: Strongylida)
    Article Snippet: .. Filters were excised from the Transwell assembly, stained using a Diff Quick kit (Dade-Behring A.G., Duedingen, Switzerland) and mounted on glass slides in Mowiol® mountant (Calbiochem). ..

    Article Title: Effect of Multi-Walled Carbon Nanotubes in the Snail Cornu aspersum
    Article Snippet: .. Diff-Quick® Kit was purchased from Dade Behring (Newark, NJ, USA), while the other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) at reagent grade. ..

    Incubation:

    Article Title: Myricetin and naringenin inhibit human squamous cell carcinoma proliferation and migration in vitro.
    Article Snippet: Myricetin and Naringenin Inhibit Human Squamous Cell Carcinoma Proliferation and Migration In Vitro Daniele Maggioni, Gabriella Nicolini, Roberta Rigolio, Luisa Biffi, Lorenzo Pignataro, Renato Gaini & Werner Garavello a Department of Surgery and Translational Medicine, University of Milan-Bicocca, Monza, Italy b Department of Otorhinolaryngology, Head and Neck Surgery, San Gerardo Hospital, University of Milan-Bicocca, Monza, Italy c Department of Clinical Sciences and Community Health, University of Milan, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy Published online: 25 Sep 2014.

    Article Title: A novel regulator of angiogenesis in endothelial cells: 5-hydroxytriptamine 4 receptor
    Article Snippet: .. After 4 h of incubation, the filter was fixed and stained with Diff Quick kit (Dade Behring, Newark, DE). ..

    Membrane:

    Article Title: Myricetin and naringenin inhibit human squamous cell carcinoma proliferation and migration in vitro.
    Article Snippet: Myricetin and Naringenin Inhibit Human Squamous Cell Carcinoma Proliferation and Migration In Vitro Daniele Maggioni, Gabriella Nicolini, Roberta Rigolio, Luisa Biffi, Lorenzo Pignataro, Renato Gaini & Werner Garavello a Department of Surgery and Translational Medicine, University of Milan-Bicocca, Monza, Italy b Department of Otorhinolaryngology, Head and Neck Surgery, San Gerardo Hospital, University of Milan-Bicocca, Monza, Italy c Department of Clinical Sciences and Community Health, University of Milan, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, Milan, Italy Published online: 25 Sep 2014.



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    diff quick stain kit  (Dade Behring)
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    Dade Behring diff quick stain kit
    Diff Quick Stain Kit, supplied by Dade Behring, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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    diff quick stain kit  (Sysmex Corporation)
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    RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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    diff quick staining kit  (Sysmex Corporation)
    86
    Sysmex Corporation diff quick staining kit
    RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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    Image Search Results


    RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

    Journal: Cancer Cell International

    Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

    doi: 10.1186/s12935-026-04262-x

    Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

    Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

    Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction